Austin FC | Colorado Rapids | FC Dallas | Houston Dynamo | LA Galaxy | Los Angeles FC | Minnesota United | Portland Timbers | Real Salt Lake | San Diego FC | San Jose Earthquakes | Seattle Sounders | Sporting Kansas City | St. Louis City | Vancouver Whitecaps

Eastern Conference:
Atlanta United | CF Montréal | Charlotte FC | Chicago Fire | FC Cincinnati | Columbus Crew | D.C. United | Inter Miami | Nashville SC | New England Revolution | New York City | New York Red Bulls | Orlando City | Philadelphia Union | Toronto FC

Former clubs:
Miami Fusion | Tampa Bay Mutiny | Chivas USA

Abandoned expansion:
Sacramento Republic FC

Related:
Central Division | eMLS | MLS Cup | MLS Cup Playoffs | MLS All-Star Game | MLS Direct Kick | MLS GO | MLS Is Back Tournament | MLS Next | MLS Next Pro | MLS Season Pass | Leagues Cup | U.S. Open Cup