Austin FC | Colorado Rapids | FC Dallas | Houston Dynamo | LA Galaxy | Los Angeles FC | Minnesota United | Portland Timbers | Real Salt Lake | San Diego FC | San Jose Earthquakes | Seattle Sounders | Sporting Kansas City | St. Louis City | Vancouver Whitecaps

Eastern Conference:
Atlanta United | CF Montréal | Charlotte FC | Chicago Fire | FC Cincinnati | Columbus Crew | D.C. United | Inter Miami | Nashville SC | New England Revolution | New York City | New York Red Bulls | Orlando City | Philadelphia Union | Toronto FC

Former clubs:
Miami Fusion | Tampa Bay Mutiny | Chivas USA

Abandoned expansion:
Sacramento Republic FC

Related:
Central Division | eMLS | MLS Cup | MLS Cup Playoffs | MLS All-Star Game | MLS Direct Kick | MLS GO | MLS Is Back Tournament | MLS Next | MLS Next Pro | MLS Season Pass | Leagues Cup | U.S. Open Cup

Western Conference:
Austin Bold FC | Colorado Springs Switchbacks | El Paso Locomotive FC | FC Tulsa | Las Vegas Lights FC | LA Galaxy II | New Mexico United | Oklahoma City Energy FC | Orange County SC | Phoenix Rising FC | Portland Timbers 2 | Real Monarchs | Reno 1868 FC | Rio Grande Valley FC Toros | Sacramento Republic | San Antonio FC | Tacoma Defiance

Future clubs:
Monterey Bay FC | Queensboro FC | Rhode Island FC

Former clubs:
FC Cincinnati | Fresno FC | Nashville FC | Penn FC | Richmond Kickers | San Diego Loyal SC | Toronto FC II

Related:
U.S. Open Cup
United Soccer League (USL League 1 | USL League 2)